Affymetrix

Affymetrix® EXP Data File Format


EXP FILE

 

Description
The EXP file contains information captured in the Experiment window of the MAS 5 software. Experiment files are not available in GCOS or LIMS. Experiment information in those applications are stored in a database and are available via the Affymetrix GCOS SDK.

Format
The format of the EXP file is an ASCII text file similar to the Windows INI format.

The first line defines the file type. The value is always: "Affymetrix GeneChip Experiment Information".

The second line contains the version number of the file. The value is always: "Version 1".

The remainder of the file is divided up into sections. The start of each section is defined by a line containing a section name enclosed in square braces. The section names are: "Sample Info", "Fluidics" and "Scanner". The data in each section is of the format TAG<tab>VALUE, where the TAG and VALUE are separated by a tab.

The "Sample Info" section contains information about the experiment. The TAGS are:

TAGDescriptionMAS Term (as displayed in the Experiment window)
Chip TypeThe type of probe array. This is the only required field.Probe Array Type
Chip LotThe lot number of the array.Probe Array Lot
OperatorThe name of the user.Operator Name
Sample TypeThe type of sample.Sample Type
DescriptionA description of the sample.Sample Description
ProjectThe project name.Sample Project
CommentsComments about the experiment.Comments
Solution TypeThe reagents used in the experiment.Reagents
Solution LotThe lot number of the reagents.Reagent Lot

The "Fluidics" section contains information about the last fluidics run. The TAGS are:

TAGDescriptionMAS Term (as displayed in the Experiment window)
ProtocolThe name of the fluidics protocol run on the fluidics station.Protocol
StationThe station number.Fluidics Station
ModuleThe module number.Module
Hybridize DateThe date and time of the hybridization.Date

Other tags may be in this section. The names of the tags are the steps in the fluidics script executed on the experiment. Other lines in this section may be defined but do not follow the TAG<tab>VALUE format. These lines may include status messages provided by the fluidics station.

The "Scanner" section contains information about the last scan. The TAGS are:

TAGDescriptionMAS Term (as displayed in the Experiment window)
Pixel SizeThe pixel size used.Pixel Size
FilterThe filter used.Filter
Scan Temperature This item is not used by the current GeneChip scanners. Temperature
Scan DateThe date and time of the scan.Date
Scanner IDThe ID of the scanner.Scanner ID
Number of ScansThe number of multi-image scans.# of scans
Scanner TypeThe type of scanner.NA